|
KMID : 1005220060210020121
|
|
Journal of Embryo Transfer
2006 Volume.21 No. 2 p.121 ~ p.128
|
|
|
Effect of the Addition of Polyvinylpyrrolidone on In Vitro Development and Cell Number of Porcine Embryo after In Vitro Fertilizatin
|
|
Park Yong-Soo
Kim Jae-Young
|
|
|
Abstract
|
|
|
|
In this study, we examined the effects of molecular weight, concentrations and treat the duration of polyvinylpyrrolidone (PVP) in vitro maturation (IVM) medium (Experiment 1), and the effect of PVP in IVM, in vitro fertilization (IVF) and in vitro culture (IVC) medium on the development and cell number of porcine embryos (Experiment 2). The base mediums were NCSU 23 solution for IVM, mTBM solution for IVF and PZM3 solution for IVC. In experiment 1, the development rates to 2 cell and blastocyst stage were not differ from the different molecular weight (MW), concentration and duration of PVP in IVM medium. However, the hatching rate of blastocyst was significantly higher in the group of MW 40,000, 0.5% and $0{\sim}44hr$ than in the other groups (p<0.05). In experiment 2, the results of IVM, IVF and IVC medium with (W) or without (W/O) 0.5% MW 40,000 PVP are follows. The development rate to 2 cell stage was highest in the group of W-W/O-W (p<0.05). The development rate to blastocyst and hatching rate was higher in the group of W-W/O-W and W-W/O-W/O than that of other treatments (p<0.05).
|
|
|
KEYWORD
|
|
|
pig, polyvinylpyrrolidone, in vitro development, cell number
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|